Ufasomes are self-assembling vesicular systems made of unsaturated fatty acids and their ionized forms are a possible substitute for traditional lipid-based drug delivery techniques. These vesicles have special physicochemical characteristics, such as outstanding biocompatibility, structural flexibility and the capacity to encapsulate both hydrophilic and lipophilic medicinal molecules. Under carefully regulated pH conditions, they grow on their own. Over the past few decades, there has been a substantial advancement in our understanding of the mechanisms behind ufasome production, stability, membrane dynamics and permeability. Recent studies show their potential in transdermal, ocular, oral and targeted drug administration due to their improved penetration, higher drug bioavailability and decreased toxicity. However, challenges such oxidative degradation sensitivity, low long-term stability and scaling-up problems continue to restrict their clinical use.