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VOL. 10, ISSUE 2 (2025)
Therapeutic evaluation of the epiphytic orchid Acampe ochracea: Antioxidant and Anti-inflammatory assays using in vivo and in vitro regenerated plantlets
Authors
Marzia Akter Sumi, Awishik Tripura, Somaya Emrog Nayma, Minhajur Rahman and Tapash Kumar Bhowmik
Abstract
The epiphytic orchid Acampe ochracea (Lindl.) Hochr. is
traditionally utilized for various medicinal purposes, yet its therapeutic
potential remains scientifically under explored, especially concerning plant
materials sourced from in vitro culture. The seeds of A. ochracea
were germinated in vitro on agar solidified four basal media viz.
KC, MS, MVW and PM and produce a substantial number of in vitro
plantlets, which were subsequently used for bioactivity assessments. This study
comparatively evaluated the antioxidant and anti-inflammatory activities of
methanolic crude extracts derived from in vivo plant parts (leaf, stem,
root) and in vitro plantlets. Antioxidant capacity was determined using
the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and
anti-inflammatory activity was assessed by measuring the inhibition of heat induced
albumin denaturation. In the DPPH assay, all samples exhibited significant
radical scavenging activity, with the natural stem extract showing high inhibition
at 250 μg/ml, closely followed by the root and in vitro plantlets. The
natural leaf extract, however, showed lower activity compared to other parts.
For anti-inflammatory testing at 250 μg/ml, the natural root extract and the in
vitro plantlet extract demonstrated the highest inhibition of protein
denaturation (57.89 ± 0.002%), comparable to each other but significantly
higher than the natural leaf (5.3 ± 0.003%). These findings demonstrate that in
vitro generated A. ochracea plantlets possess comparable, if not
superior, anti-inflammatory potential to the most active natural root part.
This research validates the traditional use of A. ochracea and supports
the utility of tissue culture as a sustainable, year-round source for
bioprospecting of bioactive compounds.
Pages:85-89
How to cite this article:
Marzia Akter Sumi, Awishik Tripura, Somaya Emrog Nayma, Minhajur Rahman and Tapash Kumar Bhowmik "Therapeutic evaluation of the epiphytic orchid <i>Acampe ochracea</i>: Antioxidant and Anti-inflammatory assays using <i>in vivo</i> and <i>in vitro</i> regenerated plantlets". International Journal of Pharmaceutical Science and Research, Vol 10, Issue 2, 2025, Pages 85-89
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