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VOL. 10, ISSUE 2 (2025)
Combined in vitro and computational study on the anthelmintic activity of the aqueous ethanolic root extract of Ocimum gratissimum
Authors
Sagar A*, Yash M, Shalini T D, Vinay Kumar R, Dr. Jose Gnana Babu
Abstract
This Research investigated the anti-helminthic activity of aqueous
ethanolic Ocimum gratissimum root extract through in silico
molecular docking and In-vitro Worm paralysis and death assay study. The
fresh plant root part was carefully collected, properly identified, and
authenticated, was thoroughly washed, shade-dried, and then coarsely ground
into a fine powder. The dried powder was subjected to hot extraction method
using the Soxhlet extraction technique with aqueous: ethanol (2:8) as the solvent.
Subsequently, the presence of phytochemical constituents was determined using
standard qualitative chemical tests. Further molecular docking was done using
5JLE protein carried by TLC for better binding score compounds. Later in-vitro
worm paralysis and death assay study were carried out using 5groups of Indian
earthworms (Pheretima posthuma) each group with 2 worms of same size and
length for plant extract and one group for Standard drug (Albendazole).
Collected earthworms were washed with normal saline (9%w/v) to remove all the
dirt matter and waste surrounding their body,10ml different concentrations of
plant Ocimum gratissimum root extract (25, 50, 100, 150, 200mg/ml) were
used and compared with 25mg/ml Std drug Albendazole, tested to determine the
time of paralysis and death of worms, while the negative control received
saline solution. The results revealed a concentration-dependent increase in
antihelminthic activity, as indicated by the progressive reduction in both
paralysis and death times with increasing extract concentration. The highest
concentration (200 mg/mL) produced paralysis and death within 21 min and 24.5
min respectively, which were comparable to that of Albendazole (26.5 min and
34.5 min). Statistical analysis using the unpaired t-test showed that the lower
concentrations (25–100 mg/mL) exhibited significant differences (p < 0.05)
compared to the standard, while higher concentrations (150–200 mg/mL) showed no
significant difference (p > 0.05), confirming similar efficacy to the standard
drug. The study concludes that the plant extract possesses potent
antihelminthic activity in a dose-dependent manner and could serve as a
promising natural alternative to synthetic anthelmintic agents.
Pages:61-66
How to cite this article:
Sagar A*, Yash M, Shalini T D, Vinay Kumar R, Dr. Jose Gnana Babu "Combined <i>in vitro</i> and computational study on the anthelmintic activity of the aqueous ethanolic root extract of <i>Ocimum gratissimum</i>". International Journal of Pharmaceutical Science and Research, Vol 10, Issue 2, 2025, Pages 61-66
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