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VOL. 10, ISSUE 2 (2025)
A comprehensive review on mass spectrometry
Authors
Rekulapally Nikhitha Reddy, Narender Boggula
Abstract
Mass spectrometry is an instrumental technique
in which sample is converted into rapidly moving positive ions which are then
separated and characterized. In
mass spectrometer ions are produced from the sample, generally by electron
bombardment. Mass spectroscopy is an analytical technique that identifies
biomolecules or proteins present in biological samples and is also useful for
studies on protein-protein interactions. The basic principle involves the
fragmentation of a compound or molecule into charged species, which are
accelerated, deflected, and finally focused on a detector according to their
mass and charge ratio. Ion deflection is based on charge, mass, and velocity,
ions separation is based on mass to charge (m/z) ratio, and detection is
proportional to abundance of ions. This chapter further discusses the back
ground, principle, major components, applications, and clinical significance of
mass spectrometry. It is the main analytical tool measuring of molecular weight
of sample. Advanced mass spectrometry methods can unambiguously identify more
than 2,000 proteins in a single proteome.
Pages:41-45
How to cite this article:
Rekulapally Nikhitha Reddy, Narender Boggula "A comprehensive review on mass spectrometry". International Journal of Pharmaceutical Science and Research, Vol 10, Issue 2, 2025, Pages 41-45
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