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VOL. 9, ISSUE 3 (2024)
Review article on high-performance liquid chromatography
Authors
Shaik Sameer, Khadar Basha Showkath Basha, Shaik Mubina Begum, Shaik Shabreen Begum
Abstract
High performance liquid chromatography is an
important qualitative and quantitative technique, generally used for estimation
of pharmaceutical and biological samples. Its properties mainly depends on
affinity,polarity and adsorption.High performance liquid chromatography employs
a liquid mobile phase that passes through a column packed with a stationary
phase,facilitating the separation of analytes based on their interactions with
the stationary phase and mobile phase.It is the most
versatile,safest,dependable and fastest chromatographic technique for the
quality control of drug components.High performance liquid chromatography
provides a highly specific,reasonable prices and fairly rapid analytical method
for a complicated samples.HPLC method in development and validation plays important
role in new discovery. It gives various validations like accuracy, precision, specificity,
linearity, range and limit of defective, limit of quantification and system
suitability testing. It also used in various industries like pharmaceutical
analysis, environmental sciences, quality control, quality research, clinical
trails, pharmaceutical chemistry. The technique is capable of analyzing a broad
spectrum of substances, ranging from small organic molecules to large
biological micro molecules, by utilizing different column types, mobile phase
compositions and detection methods.
Pages:26-31
How to cite this article:
Shaik Sameer, Khadar Basha Showkath Basha, Shaik Mubina Begum, Shaik Shabreen Begum "Review article on high-performance liquid chromatography". International Journal of Pharmaceutical Science and Research, Vol 9, Issue 3, 2024, Pages 26-31
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