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VOL. 9, ISSUE 3 (2024)
Formulation of a novel anti-inflammatory balm using Neolitsea cassia (L.)
Authors
P M Kumarapperuma, S A S D Senanayake, M M P M Hasanthi, N T B Dias, S K Hettihewa
Abstract
Inflammation is a pathological condition that may lead to various
chronic diseases. This study evaluates the anti-inflammatory effect of Neolitsea
cassia plant leaves, screens its preliminary phytochemistry, and the plant
material is subsequently used in a herbal balm formulation. The in vitro
anti-inflammatory activity of 70% acetone extract of the plant was assessed
using the human red blood cell membrane stabilization method, specifically
against heat-induced haemolysis. Aspirin was used as the positive standard of
the assay. The results demonstrated that the test plant contains various
phytochemicals such as phenolics, flavonoids, alkaloids, and saponins but no
carbohydrates. The extract exhibits significant in vitro activities
(p<0.05) in doses ranging from 125 μg/mL to 2000 μg/mL compared to normal
saline control. The bioactivity was found to be concentration-dependent and
2000 μg/mL concentration of plant extract showed the highest inhibition
percentage of in vitro haemolysis (70.35±0.01%). The balm formulations
developed with different doses of N. cassia extracts showed consistent
physical stability over two months of period from the manufacture. Therefore,
it is concluded that Neolitsea cassia plant possesses significant
anti-inflammatory activity, thus it is useful in the development of stable
anti-inflammatory herbal balm formulations.
Pages:5-8
How to cite this article:
P M Kumarapperuma, S A S D Senanayake, M M P M Hasanthi, N T B Dias, S K Hettihewa "Formulation of a novel anti-inflammatory balm using Neolitsea cassia (L.)". International Journal of Pharmaceutical Science and Research, Vol 9, Issue 3, 2024, Pages 5-8
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