Background: Rifampin, also known as Rifampicin, belongs to the antimicrobial class of drugs. This medication is used to manage and treat diverse mycobacterial infections and gram-positive bacterial infections.

Objective: A simple and sensitive Visible Spectrophotometric method has been developed for the quantitative estimation of Rifampicin in its bulk and pharmaceutical dosage form.

Method: The proposed work was carried out on a TG Ultraviolet visible spectrophotometer, T-60 Model, which is a double beam double detector configuration with a 1 cm quartz matched cell.

Results: The drug showed maximum absorbance at 475nm. Regression analysis of Beer’s plot showed correlation of 0.999 in the concentration range of 10-30μg/ml and the percentage recovery were between 98-102% indicting high degree of accuracy. The % RSD of intra-day and inter-day precision was found to be less than 2 as per ICH guidelines. Sensitivity of the method was confirmed by accurate LOD and LOQ values which were obtained 0.569 and 1.770 respectively.