ARCHIVES
VOL. 9, ISSUE 2 (2024)
Antidiabetic activity of Abies Alba seeds using In vitro starch iodine method
Authors
Ranjeet Kumar Jha, Mukesh Suniya, Monika Bansal, Jaspreet Kaur
Abstract
Abies alba
has long tradition of use in natural based medicine system of Greece for the
treatment of cancer and diabetes. Few scientific studies revealed anticancer
and antibacterial potential of the plant but traditional claims for
antidiabetic activity have not been validated. Thus, it was considered
worthwhile to investigate A. alba seeds for antidiabetic activity. Seeds
of plant were extracted utilizing solvents in expanding request of polarity viz., petroleum ether,
chloroform, methanol, and water. The chloroform, methanol and water extracts
were subjected to screen in vitro antidiabetic activity using starch
iodine method via inhibition of α-amylase enzyme. Among different extracts, the
methanol extract showed huge antidiabetic action as compared to other tested
samples. The extract showed greatest action was additionally fractionated
successively using solvents in order of increasing polarity viz., ethyl
acetate and 1-butanol for further purification and subjected to in vitro
antidiabetic activity using starch iodine method via inhibition of α-amylase
enzyme. The ethyl acetate showed huge antidiabetic action as compared to other
tested samples. Our preliminary phytochemical investigations showed presence of
flavonoids and phenolic compounds in the bioactive ethyl acetate fraction. Further,
exhaustive finding of literature suggested that a number of flavonoids and
phenolic compounds are scientifically reported for antidiabetic activity. Thus,
finally it can be suggested that these phenolic compounds and flavonoids might
be main constituents which are responsible for antidiabetic activity of plant
seeds. These antidiabetic compounds will be isolated from bioactive ethyl
acetate fraction using chromatographic techniques in future investigations.
Pages:24-29
How to cite this article:
Ranjeet Kumar Jha, Mukesh Suniya, Monika Bansal, Jaspreet Kaur "Antidiabetic activity of <i>Abies Alba</i> seeds using <i>In vitro</i> starch iodine method". International Journal of Pharmaceutical Science and Research, Vol 9, Issue 2, 2024, Pages 24-29
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