Asclepias exaltata is an herbaceous, tall perennial milkweed that got its name for its milky sap that contains latex, alkaloids and other compounds and are frequently found on the edges of forests. It resembles the common milkweed Asclepias syriaca and has been known to hybridize with this species in areas where both exist in warm relationship. Like other medicinal plants poke milkweed have highest significance for its valuable secondary metabolites (tannins, glycosides and saponins).They are known since ancient times for its curative properties and have been utilised for the treatment of various ailments such as tumor, asthma, fever, diarrhoea, gonorrhoea and warts. Hence, the present study has therefore been carried out to investigate different phytochemicals present in seed of different solvent (ethanol, chloroform, petroleum ether) extracts by employing well diffusion techniques. The phytochemical screening of seeds revealed the presence of steroids, glycosides, phenols, tannins and saponins. This experimental analysis established a best support for the use of this plant in herbal medicine and a step ahead to open a new insight for its therapeutic efficacy.