A simple, sensitive, high-throughput and robust method for estimation of candesartan in human plasma using LC-MS/MS was developed and validated.200 µL plasma samples were acidified and subjected to liquid-liquid extraction with tert-butyl-methyl-ether: dichloromethane (TBME: DCM-60:40). Dried residue is reconstituted and 5 µL of the sample is injected onto LC-MS/MS. Candesartan is separated on ACE C18, 50 x 4.6 mm, 3 µ column at a flow rate of 0.5 mL/min with a total run time of 2.0 mL/min and a single SRM transition (m/z: 441.100à263.080) was used to quantify the analyte from 1-490 ng/mL. Calibration curve standards between 1-490 ng/mL range showed good linearity with a correlation coefficient (r² > 0.993) in 5 PA batches. % CV for both inter and intra-day was less than 9%. % Nominal for inter and intra-day was between 98.52-105.21. Mean recovery is 62.57 % for candesartan and the IS normalized matrix factor was 1.02.