International Journal of Pharmaceutical Science and Research

International Journal of Pharmaceutical Science and Research


International Journal of Pharmaceutical Science and Research
International Journal of Pharmaceutical Science and Research
Vol. 5, Issue 1 (2020)

A simple, sensitive, high-throughput and robust method for estimation of candesartan in human plasma using LC-MS/MS


A Chaitanya Krishna, Dr. Cheepurupalli Prasad, Rajaram S Patil

A simple, sensitive, high-throughput and robust method for estimation of candesartan in human plasma using LC-MS/MS was developed and validated.200 µL plasma samples were acidified and subjected to liquid-liquid extraction with tert-butyl-methyl-ether: dichloromethane (TBME: DCM-60:40). Dried residue is reconstituted and 5 µL of the sample is injected onto LC-MS/MS. Candesartan is separated on ACE C18, 50 x 4.6 mm, 3 µ column at a flow rate of 0.5 mL/min with a total run time of 2.0 mL/min and a single SRM transition (m/z: 441.100à263.080) was used to quantify the analyte from 1-490 ng/mL. Calibration curve standards between 1-490 ng/mL range showed good linearity with a correlation coefficient (r2 > 0.993) in 5 PA batches. % CV for both inter and intra-day was less than 9%. % Nominal for inter and intra-day was between 98.52-105.21. Mean recovery is 62.57 % for candesartan and the IS normalized matrix factor was 1.02.
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How to cite this article:
A Chaitanya Krishna, Dr. Cheepurupalli Prasad, Rajaram S Patil. A simple, sensitive, high-throughput and robust method for estimation of candesartan in human plasma using LC-MS/MS. International Journal of Pharmaceutical Science and Research, Volume 5, Issue 1, 2020, Pages 01-05
International Journal of Pharmaceutical Science and Research