The study was conducted to investigate the anthelmintic activities of Piper betel leaves. The extract was subjected to assay for anthelmintic activity with the determination of paralysis time and death time using earthworm (Pheretima posthuma) at four different concentrations. In this study the anthelmintic activity of extract of leaves of Piper betel Linn was performed. Indian adult earthworms were used for the assessment of anthelmintic activity. Piperazine citrate (10 mg/ml) was used as standard and normal saline water was used as vehicle respectively. Observations were made for the time taken to paralysis and death.