In-vitro pharmacological screening of alkaloids from the leaves of Daphniphyllum longeracemosa as an antifungal
Harikesh Maurya, Gaurav Upadhyay
This study was designed as In-vitro pharmacological screening of alkaloids from the leaves of Daphniphyllum longeracemosa as an antifungal agent. The aqueous and methanol extracts of fresh dried leaves were obtained and subjected for preliminary phytochemical screening and TLC for the determination of phytoconstituents. The dried extract powder was found to be 10 gm and 15 gm respectively and the phytoconstituents like alkaloids, glycosides, steroids, saponins, flavonoids, phenolic compounds, proteins and amino acid were present in the extract. The In-vitro antifungal activity different strains were exposed to the plant extracts with known antifungal as positive control by the disc diffusion method and the MIC through the broth dilution method. The methanol extract showed higher inhibitory activity (26.25mm) followed by water extract (08.75mm) and standard drug (27.50mm) against the fungus, Aspergillus fumigatus. The methanol extract and positive control group also showed significant inhibitory activity against Candida albicans and Aspergillus niger respectively,which was ranging between 23.50mm to 15.60mm, and 25.45mm to 22.00mm respectively. However, the water extract showed moderate activity against the fungal species viz., Candida albicans, Aspergillus fumigates, and Aspergillus niger, respectively. The alcoholic and aqueous leaf extracts exhibited remarkable antifungal activity in respect to MIC, which was ranging between 300-400 μg/mL and 200-300 μg/mL respectively. Finally it was concluded that the new alkaloids of Daphniphyllum with unprecedented skeletons have made a great contribution to resist the growth of microbes.