In vitro protocol optimization for micro-propagation of turmeric (Curcuma Longa L.) Variety of dame via sprout bud culture
Yeshambel Emewodih, Berihu Mengs
Turmeric (Curcuma longa L.) is an Asian native species used for ornamental, medicinal and food purposes. However, its conventional propagation method is costly and inefficient. Therefore, this study aimed to verify appropriate asepsis methods for shoots from rhizomes fortified with Murashige and Skoog (MS) medium, 3% sucrose, 0.7% plant propagated agar, plant hormones and the pH was adjusted at 5.75 for turmeric growth. Rhizomatous explants from two months old buds were cultured on Murashige and Skoog’s (MS) medium supplemented with different concentrations of cytokinins and auxins for micro-propagations of turmeric cultivar. The highest plant height of 7.9 cm was recorded at growth regulator 1mg/L BA (Benzyl Adenine) combined with 0.25 mg/L NAA (α-Naphthalene Acetic Acid). Whereas the highest yield of shoots 23.3 were obtained from 2mg/L BA combined with 1mg/L NAA. In addition to that, the highest root length of 5.1 cm and yield of root number 13.6 were obtained from 0.5mg/L NAA as compared to the control. Hence the plant growth regulator have been grate tendency to hardening off in the green house on the soil media of top soil, coffee husk and sand at the ratio 2:1:1respectively were 100% survival as compared to Top soil: vermin compost: sand at 2:1:1 ratio respectively 90% survival has been showed.