Natural products form one avenue in the search for new anti-tubercular agents with many groups undertaken screening of natural products as the preliminary step to finding new lead compounds. The anti-TB activity of ethno pharmacologically used Sterculia setigera leave was investigated by bioactivity-guided fractionation against virulent strains of Mycobacterium tuberculosis (H₃₇Rv (ATCC27294)) in-vitro, using the Alamar Blue Assay. Repeated purifications of the active fractions using combination of normal and reverse phase chromatography led to the isolation of a fraction with an interesting anti-TB activity (minimum inhibitory concentration of 15.13 µg/ ml). Preliminary spectroscopic studies shows that the most active fraction is a mixture of isomeric glycolipids based on evidence obtained from ¹H, ¹³C nmr spectra. Effort is currently ongoing to separate the mixture for complete individual structure elucidation. This preliminary report is evident to the fact that glycolipids are the anti-mycobacterial agents in S. setigera leaves. This is the first report on the occurrence of biologically active glycolipids from S. setigera leaves.