Sanjeevi theeneer (ST) is a novel siddha distillate formulation used mainly in conditions of Jaundice, and Cardiac diseases. Previous preliminary phytochemical studies on the drug had shown the presence of phenolic compounds that are natural radical scavengers. The present study aimed to investigate the antioxidant activity of Sanjeevi theeneer (ST) against DPPH (2, 2-diphenyl 1-2 picrylhydrazyl) free radical. 1 ml of 0.3 mM DPPH methanol solution was added to 2.5 ml of sample solution of different concentrations and allowed to react at room temperature. Absorbance was read out at 517 nm using double-beam U.V Spectrophotometer. The effective concentration of test sample ST required to scavenge DPPH radical by 50% (IC₅₀ value) was obtained as 90.19 ± 8.57 μg /ml as compared with Ascorbic acid (46.91± 9.93 μg /ml) showing its Intermediate anti-oxidant activity. The report of the present assay supports the Anti-oxidant potential of Sanjeevi theeneer for its wide clinical applications.