Aim: In this study we will see about the source, extraction, co-constitutes of papain especially from Carica papaya which is actively used nowadays to get symptomatic relief from dengue fever. We will also discuss about the extracts and formulations that can be done using Carica papaya leaves so that we get a maximum extent of papain activity. We focus on papain because of its broad range of activity than other constituents which have lesser activity that are found in Carica papaya.