International Journal of Pharmaceutical Science and Research

Authors
Rida Aslam Lodhi, Faqir Muhammad, Bilal Aslam, Damião Pergentino de Sousa, Ahmad Raza, Faiza Naseer

Abstract
Investigations of cytotoxic and mutagenic effects of limonene 1, 2-epoxide (LE), hydroxycitronellal (HC), thymyl acetate (ThA), terpinyl acetate (TrA), and isopulegone (IS) were carried out. The cytotoxicity was determined with hemolytic assay and brine shrimp lethality bioassay (BSLB) while mutagenicity was determined by using Salmonella typhimurium TA100 and TA98 strains. Hemolytic activity of LE, HC, ThA, TrA and IS was 13, 3.07, 10.63, 22.1 and 8.73% respectively, at a concentration of 10 mg mL^-1 in comparison to the control. The median lethal concentration (LC₅₀) values for these compounds as calculated with probit method were 7.95, 1.05, 6.38, 3.92 and 4.79 mg mL^-1, respectively. All the tested compounds showed non-mutagenic activity to S. typhimurium TA100 and TA98 strains. The present investigation suggested that all the tested compounds are also non-toxic to human RBCs and brine shrimp larvae so; these chemicals might be safely used for pharmaceutical benefits.