The objective of this study was to evaluate the antidiabetic activity of silver nanoparticles (SNPs) in a model of dextrose -induced experimental diabetes in rats. Silver nanoparticles were used as reducing agent. In control and hyperglycemic rats was performed the measurement of blood glucose levels and glucose tolerance tests. Hepatic Glycogen Synthase enzymes were decreased from 41.07% to 26.20% when diabetic rats treated with silver nanoparticles. The protein metabolic enzymes includes Total synthesis of –L-Hexoses (5.27 mmol/L to 5.14 mmol/L), Serum Hexosamine (135.13 mg/%) to 132.95 mg/%)) and serum Sialic acids (83.70 mg/%) to73.80 mg/%) were decreased by the silver nanoparticle treatment. Intra peritoneal injection of silver nanoparticles decreased the carbohydrate and protein metabolic enzymes levels. The experiment showed the silver nanoparticles have antidiabetic activity.