The present study deals with the In Vitro callus induction of Physalis minima L. is an important medicinal plant which belongs to the family Solanaceae. It is widely used for inflammation, enlargement of the spleen and abdominal troubles. The efficient in vitro callus induction of Physalis minima was achieved from internode and leaf explants on MS medium with B5 vitamins and different concentrations and combinations of BAP, IAA, NAA and 2.4-D.The highest rate of callus induction was observed from the indrtnode and leaf explants on MS medium with BAP (3.0 mg/l) + NAA (1.0 mg/l) + 2.4-D (1.0 mg/l) and IAA (2.0 mg/l).The regenerated callus was transferred to half strength MS medium fortified with IAA (2.0 mg/l The Callus induction was successfully the current study showed efficient in vitro callus induction capabilities of Physalis minima L.