The aim of the current study was to assess Psidium Guajava ethanolic leaf extract for its phytochemical constituents and anti hemolytic activity against H₂O₂ Induced Hemolysis in chicken erythrocytes. The phytochemical investigation of ethanolic extract showed the presence of carbohydrates, flavanoids, alkaloids, and saponins. In this present study ethanolic leaf extract of Psidium guajava shown to have moderate anti hemolytic activity against H₂O₂ induced hemolysis in chicken erythrocytes. The Ethanolic extract showed antihemolytic activity in the range from 12.5% to 43.75%. At varying concentration of plant extracts 20, 40, 60, 80 and 100 mg /ml the percent of inhibition of hemolysis recorded were 12.50%, 18.75%, 25%, 37.50%, 43.75% respectively.