The present study focuses on the bioproduction of Zinc oxide nanoparticles (ZnO NPs) from the aqueous extracts of leaves, stem, roots, flowers and fruits of Melia azedarach L. The plant is enriched with alkaloids, sterols, glycosides, phenolic compounds, tannins, flavonoids, saponins and other biologically active phytocompounds. Zinc Nitrate hexahydrate [Zn (NO3)2.6H2O] was used as precursor to synthesize ZnO nanoparticles. UV- Visible spectral studies were conducted for the characterization and confirmation of the synthesized ZnO nanoparticles. It showed that the test solutions had strong absorbance in the range of 290-330 nm. Hence the biogenic synthesis of ZnO nanoparticles using M. azedarach could be explored in various fields.