RP—HPLC method development and validation for the estimation of valsartan in tablet dosage form
Mansi L Patil, Ganesh M Sanap, Shubham J Khule
A rapid, sensitive and simple RP-HPLC method was developed for the estimation of valsartan in tablet dosage form. The separation was carried out by using Agilent technology HPLC. The column used was C18 (4.6 ×1000mm) with methanol: 0.05% orthophosphoric acid as mobile phase (75:25%v/v) flow rate was 0.7ml/min. analyte was measured at wavelength of 239nm. The retention time for valsartan was found to be 3.62 min. the validation of selected method was carried out with respect to linearity, precision, accuracy, robustness and LOD & LOQ. Linearity studies were performed in the range of (5-25µg/ml). LOD and LOQ was found to be 0.060 and 0.1837 respectively. Relative standard deviation for accuracy was found to be less than two percent. Force degradation studies were carried out and drug was subjected to stress conditions of hydrolysis, oxidation, thermal degradation and photolysis.
Mansi L Patil, Ganesh M Sanap, Shubham J Khule. RP—HPLC method development and validation for the estimation of valsartan in tablet dosage form. International Journal of Pharmaceutical Science and Research, Volume 5, Issue 3, 2020, Pages 14-19