In vitro Protocol optimization for micro propagation of yam landraces (Dioscorea species) using nodal culture
Ibrahim Hussein, Berihu Mengs, Temesgen Matiwos
Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates. However, In vitro propagation is the best alternative to overcome such limitations of conventional propagation. For both shoot multiplication and rooting induction experiments, nodal segments were cultured on hormone free MS medium with combination of 30 g/l sucrose and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0.0, 0.5, 1.0, 2.0 and 2.5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0.0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l indol -3-butyric acid (IBA). Finally, for acclimatization, In vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced In vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23 ± 0.21) with a maximum shoot length7.68 ± 0.24cm and 6.79 ± 0.09 shoot number with 7.47± 0.47 cm were obtained on MS media supplemented with 1.5 mg/l BAP for 6/02 and 75/02 cultivars respectively. The maximum root number 10.03 ± 0.49 and root length10.76± 0.16 cm and 10.58 ± 0.26 root number with 10.42 ± 0.32 cm were recorded on MS media with 1.5 mg/l IBA for 75/02 and 6/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in75/02 and 6/02, respectively, on soil medium with combination ratio of 1:2:1 top soil, sand soil and compost, respectively.
Ibrahim Hussein, Berihu Mengs, Temesgen Matiwos. In vitro Protocol optimization for micro propagation of yam landraces (Dioscorea species) using nodal culture. International Journal of Pharmaceutical Science and Research, Volume 4, Issue 3, 2019, Pages 31-38