In the present study a screening design of experiments was applied in preliminary evaluation of oral suspension formulation of Rifampicin. Rifampicin is a widely used antibiotic to treat a several types of bacterial infections like tuberculosis, leprosy, and Legionnaire's disease. A Plackett-Burman screening design was used to identify significant variables affecting critical quality attributes (CQAs). Impact of formulation and manufacturing process variables like surfactant (%), hydrocolloid (%), homogenization speed (rpm) and homogenization time (min) was studied on critical quality attributes (CQAs) of Rifampicin oral suspension. Viscosity (cps) and content uniformity (% C.V.) were identified as CQAs of Rifampicin oral suspension.