In vivo anxiolytic activity of Gynura procumbens (Asteraceae) leaves extract by gamma-aminobutyric acid (Gaba) mediated hyperpolarization in mice
Sharmin Sultana, Lokman Hossain
Gynura procumbens of Asteraceae family is a commonly found medicinal plant in Bangladesh, India, China, Thailand, Indonesia, Malaysia, and Vietnam. Traditionally, it is extensively used in different countries for the management of a wide range of disorders such as kidney discomfort, rheumatism, diabetes mellitus, constipation, and hypertension. As part of our current research of traditional medicinal plant, this study assessed the anxiolytic activity of Ethanol Extract of Gynura procumbens (EEGP) leaves. Anxiolytic activity was screened by elevated-plus maze, light-dark box, hole-board and marble-burying test method in mice at the doses of 200 and 400 mg/kg body weight where Diazepam was used as the positive control. The phytochemical analysis of EEGP also carried out by standard methods. Elevated plus maze (EPM) and light-dark box test results explicated that mice preferred open arms and light part instead of close arms and dark part significantly (p<0.001). In hole-board and marble-burying test EEGP (200 & 400mg/kg body weight) reduced (p<0.001) the number of head dipping and number of marble burying, respectively. Phytochemical screening of EEGP revealed the presence of tannin, flavonoids, steroids, glycoside, alkaloids and terpenoids. The experimental result indicates that G. procumbens contains phytoconstituents that possess anxiolytic activity which traditionally used as depression and anxiety management. So, the plant may be further subjected to chemical investigation to isolate the bioactive compound(s) responsible for its pharmacological activity.