Vol. 2, Issue 6 (2017)
Bioanalytical method development and validation of prednisolone in rat plasma using RP-HPLC method
Author(s): Kiran Nathe, Dr. Sam Soloman
Abstract: A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed and validated for quantitative determination of Prednisolone in plasma. Metformin is used as an internal standard. The method was carried out with Anaytical technologies Lts. Model no. 3000Series. The optimized chromatographic conditions were optimized using a mobile phase methanol: water in the ratio of 70:30 at flow rate 0.9ml/min. Stationary phase was used as Grace C18 column (250mm x 4.6ID, partical size:5 micron). Detection was carried out at 238nm.The method was developed and tested for linearity range of 100ng/ml to 1600ng/ml. The developed method was validated in terms of selectivity, accuracy, precision, linearity and stability study. Proposed developed method can be used in bioanalytical, bioequivalence & pharmacokinetic studies with desired precision and accuracy.