Vol. 2, Issue 1 (2017)
Evaluation of acute and sub-acute hepatotoxic activity of leaves of Ehretia laevis n-hexane extract in mice
Author(s): Nadia Perveen, Khwaja Zafar Ahmed, Qura-tul-Ain, Shaneel Kausar, Aisha Shehzad, Amber Shafi, Faiza Naseer
Abstract: Objective: The aim of the present study was to evaluate the acute and subacute hepatotoxicity of Ehretia laevis (leaves). Methods: Toxicity study of Ehretia laevis was carried out in Swiss female mice after single ingestion of the extract at different doses (acute toxicity) and daily administration for fifteen days (subacute toxicity). Results: The results showed that the LD50 of the extract was higher than 4000mg/kg and subacute treatment showed no change in weight of liver however, ALT, ALP, AST bilirubin and globulin values increased significantly whereas albumin and protein decreased. Histopathological studies showed aggregation of cells in parenchyma and in blood vessels vicinity at 1000mg/kg. At 2000mg/kg vacuolation was present, sinusoidal spaces absent, acute cellular swelling and mild degree of cell necrosis was noted. At 4000mg/kg cellular swelling was found to be increased and large number of polymorphic cells was present in blood vessels, showing necrosis. Extract showed dose related toxic effects on hepatocytes. Conclusions: On the basis of above findings it is concluded that the n- hexane extract of leaves of Ehretia laevis has hepatotoxic effect in mice. Further, chronic toxicity studies are needed in other species and with different extract.