Vol. 1, Issue 6 (2016)
Evaluation of In vitro anti-diabetic and anti-inflammatory effects of Nigella sativa seed extracts in five different solvent systems
Author(s): Hazrat belal, Ruksana Yesmin, Al Mamun, Nazmul Hasan, Dobirul Islam, Hanif Ali, Atikur Rahman, Amirul Islam
Abstract: Objective: The objective of this study was to screen the phytochemical constituents of the seeds of the Nigella sativa and to obtain preliminary in vitro data about its anti-diabetic and anti-inflammatory effects. Methods: First extracts were prepared in five solvents i.e. Methanol, ethanol, n-hexane, petroleum ether and chloroform through cold extraction procedure. Determinations of the phytochemical constituents of these extracts were then carried out by employing standard procedures. In order to determine the in vitro anti-diabetic activity, amylase inhibition studies were carried out. In vitro anti-inflammatory test were carried out using protein denaturation inhibition principle. Results: With the exception of the chloroform extract, all the other extracts were found to be rich in phytochemicals. The methanol extract contained the maximum number of phytochemicals. The alpha amylase inhibition and protein denaturation inhibition were all found to be highest for the methanol extract. Conclusion: The present study proves that the methanol extract of N. sativa a potent anti-diabetic and anti-inflammatory agent. It can prove to be a valuable source of drugs targeting these diseases.